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Linfield Home » Arts & Sciences » Chemistry » Current Course Offerings » CHEM_440

CHEM 440 - Biochemistry

Instructor: Megan Bestwick

Office: Murdock Hall room 120

Office Hours: M, W 11:00 am – 12:30 pm; Th 8:30 – 9:30 am; 
                        or by appointment

Phone: 503.883.2265

Email: mbestwic@linfield.edu

 

Course Materials

Lecture: MWF, 9:30 - 10:35 am, TAYH 102

Lab:
 F, 2:00 - 5:00 pm, MRUD 106


Lecture & Lab Syllabus

Protocols for Lab Experiments

Micropipette Use & Calibration

        - Density of Water vs. Temperature
        - Gilson Guide to Pipetting

Preparation of Buffers

Spectrophotometric Analysis

Gel filtration chromatography

Lysozyme: Isolation and Optimum pH

Kinetics of Chymotrypsin Catalysis

Lactate Dehydrogenase: Extraction, Purification & Kinetic Behavior

 
Independent Biochemistry Project


Useful Course Links

Current Lecture Schedule & Course Content

Format & Guidelines for Laboratory Reports
Examples of Biochemistry Related Scientific Papers:
          Cobine PA, et al. (2006).  J. Biol. Chem281, 36552–59.
          Liu L, et al. (2011).  J. Biol. Chem286, 41253-64.
          Bestwick ML, et al. (2010). Mol. Cell. Biol30, 172-185.
          Brown BM, et al. (2004). Biochem43, 13541-48.
          
Lehninger, Principles of Biochemistry, 6th ed: Book Companion Website
 

Useful Biochemistry Links

Titration Curve of a Weak Acid
Titration Curves for Acetic Acid, Phosphate, and Ammonia

Titration of a Polyprotic Acid

Animation: Peptide Bond Formation (1)
Animation: Peptide Bond Formation (2)

Animation: The Alpha Helix
Animation: Beta Sheets

Common Protein Chromatography Methods: Ion-exchange & Gel filtration

Protein Sequence, Stucture & Function Resources

How Enzymes Work
Enzyme Catalysis
Enzyme Kinetics
     Progress Curves for a Simple Enzyme-catalyzed Reaction 
     Double-reciprocal (Lineweaver-Burk) Plot
     Lineweaver-Burk Plot for Competitive Inhibition
     Lineweaver-Burk Plot for Uncompetitive Inhibition
     Lineweaver-Burk Plot for Mixed Inhibition

Map of Metabolic Pathways 
Interactive Metabolic Pathways Map

Glycolysis
     Animation: Overview of Glycolysis
     Interactive Animation: Glycolysis Enzyme/Reactant Name/Reactant Structure Match-up

Gluconeogenesis
     Interactive Animation: Gluconeogenesis
     Animation: The Transport of PEP and Oxaloacetate from the Mitochondrion to the Cytosol
     Comparison of the Pathways of Gluconeogenesis and Glycolysis



Summer Research Opportunities

Amgen Scholars Program for undergraduate students
Fred Hutchinson Cancer Research Center: Summer Undergraduate Research Program
Oregon Health Sciences University: Listing of various research opportunities

 

For Fun

Glucose song (YouTube video)
Bio-Rad GTCA SsoFast (YouTube video)