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Chemistry

CHEM 440 - Biochemistry

Instructor: Megan Bestwick

Office: Murdock Hall room 120

Office Hours: M, W 11:00 am – 12:30 pm; Th 8:30 – 9:30 am; 
                        or by appointment

Phone: 503.883.2265

Email: mbestwic@linfield.edu

 

Course Materials

Lecture: MWF, 9:30 - 10:35 am, TAYH 102

Lab:
 F, 2:00 - 5:00 pm, MRUD 106


Lecture & Lab Syllabus

Protocols for Lab Experiments

Micropipette Use & Calibration

        - Density of Water vs. Temperature
        - Gilson Guide to Pipetting

Preparation of Buffers

Spectrophotometric Analysis

Gel filtration chromatography

Lysozyme: Isolation and Optimum pH

Kinetics of Chymotrypsin Catalysis

Lactate Dehydrogenase: Extraction, Purification & Kinetic Behavior

 
Independent Biochemistry Project


Useful Course Links

Current Lecture Schedule & Course Content

Format & Guidelines for Laboratory Reports
Examples of Biochemistry Related Scientific Papers:
          Cobine PA, et al. (2006).  J. Biol. Chem281, 36552–59.
          Liu L, et al. (2011).  J. Biol. Chem286, 41253-64.
          Bestwick ML, et al. (2010). Mol. Cell. Biol30, 172-185.
          Brown BM, et al. (2004). Biochem43, 13541-48.
          
Lehninger, Principles of Biochemistry, 6th ed: Book Companion Website
 

Useful Biochemistry Links

Titration Curve of a Weak Acid
Titration Curves for Acetic Acid, Phosphate, and Ammonia

Titration of a Polyprotic Acid

Animation: Peptide Bond Formation (1)
Animation: Peptide Bond Formation (2)

Animation: The Alpha Helix
Animation: Beta Sheets

Common Protein Chromatography Methods: Ion-exchange & Gel filtration

Protein Sequence, Stucture & Function Resources

Enzyme Kinetics
     Progress Curves for a Simple Enzyme-catalyzed Reaction 

Map of Metabolic Pathways 


For Fun

Metabolic Pathway Songs (all MP3): glucose song (YouTube video); betaox; chemiosmotic; fattyacid; glycolysis; glyoxylate; Krebs; Michaelis; oxphos; pentose; photosynthesis; protein; urea